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KMID : 0613820100200101519
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Journal of Life Science
2010 Volume.20 No. 10 p.1519 ~ p.1524
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Studies on the Anticancer Effect of Apigenin in KB Cell Xenograft Nude Mouse Model
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Lee Jin-Seok
Seo Hyeong-Seok
Kim So-Jeong
Kim Hyeong-Jin
Kim Jin
Lee Seung-Ho
Park Young-Seok
Park Byung-Kwon
Kim Byeong-Soo
Kim Sang-Ki
Jung Ji-Youn
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Abstract
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Apigenin (4", 5, 7-trihydroxyflavone), a common dietary flavonoid abundantly present in fruits and vegetables, has shown remarkable anti-proliferative effects against various malignant cell lines. To observe the anti-proliferative effects, oral cavity cancer cell lines, 6×10³ cells/well (96 well plate) of KB oral cavity tumor cells were plated and 24 hr later treated with apigenin for one day, after which MTT assay was performed. Apigenin induced cell death in a dose-dependent manner after incubation. Cell viability was significantly decreased in the group treated with 100 μM apigenin for 24 hr (p＜0.05) compared to the control group. To assess apoptosis, the nuclei of KB cells were stained with DAPI. The presence of chromatin condensation in the apigenin treated cells was detected on a fluorescent microscope (×200). We investigated the in vivo growth inhibitory effects of apigenin on oral cavity cancer KB tumor xenograft subcutaneously implanted in male nude mice. Apigenin was administered to mice by gavage at doses of 25 and 50 ㎎/㎏/day in 0.2㎖ of PBS. Tumor volume was significantly decreased in 25 and 50 ㎎/㎏ apigenin-administration groups compared to the control group. For apoptosis analysis, TUNEL staining was performed. A significant increase in TUNEL positive cells was found in the 25 ㎎/㎏ apigenin administration group compared to the non- apigenin administration group. Histopathological changes were not observed. These results indicate that apigenin inhibits oral cavity cancer cell growth through the induction of apoptosis.
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KEYWORD
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Apigenin, oral cavity tumor cells, flavonoids, apoptosis
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